J Biomed Lab Sci 1989; 2: 201~213
ENHANCEMENT OF SPLENIC NK CYTOTOXIC ACTIVITY BY EXTRACTS OF GANODERMA
LUCIDUM MYCELIUM IN MICE
SHEN-JEU WON, SHIUH-SHENG LEE, YEN-HSIU KE and MAO-TSUN LIN
Department of Microbiolog, College of Medicine, National Cheng-Kung
Department of Biochemistry, National Yang-Ming Medical College,
Department of Physiology, Collegeof Medicine, National Cheng-Kung
University, Tainan, Taiwan, R.O.C.
The effects of alcohol insoluble fraction of Ganoderma lucidum
mycelium (GL-AI) on the splenic natural killer cell (NK) cytotoxic
activity were assessed in normal mice and in tumor bearing mice.
Intraperitoneal administration of GL-AI (4-400 mg/kg) augmented
the splenic NK cytotoxic activity in normal C3H/HeN mice in a dose-dependent
way. Augmentation of splenic NK cytotoxicity was neither mouse strain-dependent
nor route administration-dependent. For an i.p. does of 40 mg/kg
of GL-AI, the splienic NK activity effect began to rise at 15hrs
after injection and reached its peak level at 48 hrs. The splenic
NK activity returned to its normal level at 120 hrs. In addition
to an enhancement in the splenic NK activity, i.p. administration
of GL-AI also elevated the interferon (IFN) titers in mouse serum.
The decreased splenic NK cytotoxicity of 129P hepatoma-bearing C3H/HeN
or B16-F10 melanoma-bearing C57BL/6 mice could be restored by i.p.
injection of 40 mg/kg of GL-AI. However, the hyporesponsiveness
to splenic NK augmentation did develop following consecutive 3 or
more doses of GL-AI. These data indicate that GL-AI induces the
IFN production in mice, which, in turn, activates the splenic NK
activity to lyse the tumor cells.
Keywords: Ganoderma lucidium, NK cell, cytotoxicity.