出處
American Journal of Chinese Medicine, Vol.XXIII, No.1, pp. 71-80
? 1995 Institute for Advanced Research in Asian Science and Medicine
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題目
Effects of Ganoderma Lucidum and Krestin on Cellular Immunocompetence
in γ-Ray-irradiated Mice
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作者
Wang-Chi Chen, Dou-Mong Hau and Shiuh-Sheng Lee
Insitute of Radiation Biology, National Tsing Hau University, Hsinchu,
Taiwan
Department of Biochemistry, National Yang-Ming Medical College,
Taipei, Taiwan
To whom correspondence should be addressed
(Accepted for publication July 7, 1994)
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論文摘要
Abstract: The effects of Ganoderma Lucidum(Gl) and Krestin(PSK)
extracts on cellular immunocompetence, leukocyte counts and differential
count inγ-irradiated mice were investigated in this study. ICR strain
male mice were used and randomly divided into five groups. Group
A is normal control. Group B, the experimental control, was treated
with Gl. Group C, the radiation treatment control, was treated with
whole body exposure to 4 Gyγ-irradiation (RT). Group D was treated
with RT and Gl. Group E was treated with RT and PSK. The dosage
of Gl was 400 mg/day/kg body weight and PSK was 500 mg/day/kg body
weight. After irradiation, six mice from each group were sacrificed
on day 7 and the six on day 28. cellular immunocompetence was measured
by means of 3H-thymidine incorporation with splenic cells stimulated
through mitogens such as PHA, Con A and LPS. The results revealed
that relative splenic weight in Groups D and E were higher than
group C on day 28 after γ-irradiation, Group D was the highest in
all the experimental groups. Leukocyte counts were decreased significantly
in Groups D and E on day 7, the former was a little higher than
the latter. Gl administration showed an increase in the leukocyte
count in group D on day 28. The blastogenic response of splenocytes
to PHA and Con A in groups D and E were higher than in Group C on
days 7 and 28. We suggested that Gl and PSK were effective in enhancing
the recovery of cellular immunocompetence fromγ-Ray-irradiation.
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