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出處 Int. J. Cancer: 70,699-705 (1997) |
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題目 THE ANTI-TUMOR EFFECT OF GANODERMA LUCIDUM IS MEDIATED BY CYTOKINES RELEASED FROM ACTIVATED MACROPHAGES AND T LYMPHOCYTES |
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作者 Sheng-Yuan Wang, Ming-Ling Hsu, Hui-Chi Hsu, Cheng-Hwai Tzeng,
Shiuh-Sheng Lee, Ming-Shi Shiao and Chi-Kuan Ho |
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論文摘要 The present study was to ascertain the immunomodulating and anti-tumor
effects of Ganoderma(G.) lucidum. Polysaccharides (PS) from
fresh fruiting bodies of G. lucidum (PS-G) were isolated and used
to potentiate cytokine production by human monocytes-macrophages
and T lymphocytes. Our results had shown that the levels of interleukin
(IL)-Iβ, tumor necrosis factor (INF)-α,and IL-6 in macrophage cultures
treated with PS-G (100 μg/ml) were 5.1-, 9.8- and 29-fold higher,
respectively, than those of untreated controls. In addition, the
release of interferon(IFN)-γ from T lymphocytes was also greatly
promoted in the presence of PS-G (25-100μg/ml). Furthermore, these
cytokine-containing mononuclear cell-conditioned media (PSG-MNC-CM)
were found to suppress the proliferation and clonogenicity of both
the HL-60 and the U937 leukemic cell lines. DNA labeling and gel
electrophoresis showed that treatment with PSG-MNC-CM markedly induced
leukemic-cell apoptosis. Flow-cytometric analysis revealed that
few (2.3±0.8%) apoptotic cells were seen in the control cultures,
while PSG-MNC-CM treatment resulted in a significant increase in
the apoptoic population both in the HL-60(38.3±4.5%) and in the
U937(44.5±3.8%) cells. In addition, 40 to 45% of the treated leukemic
cells were triggered to differentiate into mature monocytic cells
expressing CD14 and CD68 surface antigens. However, PS-G alone had
no such effects even at a higher does of 400μg/ml. Since untreated
macrophages and T lymphocytes produced little or no cytokine, and
normal MNC-CM did not suppress leukemic cell growth, it was suggestive
that the anti-tumor activity of PSG-MNC-CM was derived from the
elevated levels of cytokines. Antibody-neutralization studies further
revealed that the anti-tumor cytokines in the PSG-MNC-CM were mainly
of INF-α and IFN-γ, and these 2 cytokines acted synergistically
on the inhibition of leukemic-cell growth. Int. J. Cancer: 70,699-705,
1997 |
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